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Day 3 embryo development rate

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Written by Andrew Manderson

What this card shows

The Day 3 embryo development rate card shows what share of normally fertilized oocytes had reached eight or more cells by Day 3, month by month.

Eight cells on Day 3 is the on-schedule milestone at this stage. It is the last cleavage-stage checkpoint before the embryo starts compacting on its way to becoming a blastocyst.

There are three lines:

  • Day 3 development rate: your monthly rate.

  • Average for selected period: your rate across the whole date range, drawn as a dashed line.

  • Competency: the Vienna Consensus competency level, 45% or above.

Average for selected period adds up every embryo at eight or more cells on Day 3 in the date range and divides by every normally fertilized oocyte graded on Day 3 in it, so a busy month counts for more than a quiet one. Compare it with the Competency line to see where your lab sits over the whole period rather than month by month. It follows every filter at the top of the dashboard.

The Vienna Consensus publishes only a competency level for this measure, not a higher benchmark, so this card carries a single reference line rather than two.

Reading the card

  • The denominator is normally fertilized oocytes (2PN at the Day 1 check) that were actually graded on Day 3 with a cell count. Embryos with no Day 3 grading are left out, as are Day 3 entries recorded as compacting, morula, degenerated, or other outcomes that are not cell counts.

  • This is a deliberate departure from the strict Vienna formula, which uses all normally fertilized oocytes as the denominator. Most labs now grade at Day 5 rather than Day 3, so the strict denominator would be dominated by embryos nobody looked at rather than by embryos that developed slowly.

  • The practical consequence: if your lab grades only a subset of embryos at Day 3, this card describes that subset. It is not a whole-cohort figure.

  • Compacting and morula entries being excluded is worth understanding. An embryo that has already begun compacting is developing well, arguably ahead of an eight-cell embryo, but it has no cell count to score, so it cannot be counted either way.

  • The Competency line is a flat reference line from the Vienna Consensus, not a measurement from your lab.

  • The current month is still in progress. Embryos fertilized in the last three days have not been graded on Day 3 yet, so the latest month, and the average with it, rests on fewer embryos until they are. Because only graded embryos count, this makes the latest month less steady rather than lower. For a settled figure, set Date to end at the last full month.

  • Hover any month to see its exact rate and the average for the period.

The card lives on the Laboratory performance (Vienna consensus KPIs) dashboard, on the Embryo Development tab, and responds to the filters at the top:

  • Date: change the time window. It scopes by the date of fertilization, and defaults to the past 6 months plus the current month.

  • Clinic: focus on one or more locations, if your organization has several.

  • Treatment type, Age at cycle start and BMI: narrow to a subset of cycles.

Where this data comes from

Both halves of this card come from the Culture tab of the patient's Cycle page: the Day 1 observation carries the pronuclei grading that puts an embryo into the cohort, and the Day 3 observation carries the cell count.

The cell count is what this card reads, so a Day 3 entry recorded without one cannot be scored. As with the Day 2 cards, consistency in the timing of the check matters: an observation logged several hours late will read as further along than a same-time comparison would.

Opening this card

In wawa, open Insights from the sidebar, then go to Standard Reports → Laboratory Performance → Laboratory performance (Vienna consensus KPIs). Select the Embryo Development tab. The Day 3 embryo development rate card is on it.

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