What this card shows
The 1PN rate (IVF) - by embryos inseminated card shows what share of inseminated oocytes showed a single pronucleus at the Day 1 fertilization check, month by month. It covers conventional IVF only, where oocytes and sperm are placed together in a dish.
A pronucleus (PN) is a visible nucleus in the newly fertilized oocyte. Two of them, 2PN, is the normal result. Just one, 1PN, is an abnormal outcome, and roughly half of these oocytes turn out to carry an abnormal chromosome complement.
There are three lines:
1PN rate: your monthly rate, counting every oocyte placed in the dish.
Excluding discarded oocytes: the same rate with discarded oocytes left out.
Benchmark: the Vienna Consensus benchmark, below 5%.
Excluding discarded oocytes is the dotted line, shown for contrast. It answers the same question while leaving out any oocyte the lab later discarded.
The two lines together tell you how much of your result rests on oocytes that were discarded afterwards. The headline is the one to read and the one the reference lines apply to, because the Vienna Consensus fixes this denominator at the Day 1 observation, before any disposal decision has been made, and it declines to set benchmarks for measures that depend on what a lab chooses to discard or freeze. The dotted line therefore has no competency or benchmark applied to it.
This is one of the cards where lower is better, so you want your line sitting below the benchmark.
Reading the card
The denominator is every oocyte placed in the IVF dish for the cycle. This is the denominator that maps directly to the Vienna formula, which is why the benchmark is drawn against this version of the card.
Oocytes that were later discarded still count, on both sides of the rate. Because an abnormally fertilized oocyte is usually discarded soon afterwards, leaving those out would hide much of what this card is looking for.
The Benchmark line is a flat reference line from the Vienna Consensus, not a measurement from your lab.
Hover any point to see the exact figure.
Because the rate is normally low, single months can look volatile when the number of inseminated oocytes is small. Read the trend across several months.
There is a companion card, 1PN rate (IVF) - by oocytes retrieved, which scores the same numerator against every oocyte collected on the cycle rather than only those inseminated. It always reads lower. Use this version for benchmark comparison, and the other for a whole-cycle view.
A sustained rise above the benchmark points to something in gamete handling or culture conditions rather than to chance.
The card lives on the Laboratory performance (Vienna consensus KPIs) dashboard, on the Fertilization Performance tab, and responds to the filters at the top:
Date: change the time window. It scopes by the date of fertilization, and defaults to the past 6 months plus the current month.
Clinic: focus on one or more locations, if your organization has several.
Treatment type, Age at cycle start and BMI: narrow to a subset of cycles.
Where this data comes from
Both halves of this card come from the Culture tab of the patient's Cycle page: the insemination is recorded as a fertilization event, and the Day 1 observation on each oocyte carries the pronuclei grading.
Recording the pronuclei count on every inseminated oocyte, including the abnormal ones, is what makes this rate accurate. An ungraded oocyte counts in the denominator but can never appear in the numerator, so gaps in recording push this rate down rather than up.
Opening this card
In wawa, open Insights from the sidebar, then go to Standard Reports → Laboratory Performance → Laboratory performance (Vienna consensus KPIs). Select the Fertilization Performance tab. The 1PN rate (IVF) - by embryos inseminated card is on it.