Skip to main content

IVF normal (2PN) fertilization rate

A
Written by Andrew Manderson

What this card shows

The IVF normal (2PN) fertilization rate card shows what share of eggs fertilized normally in conventional IVF, month by month. It covers conventional IVF only, where eggs and sperm are placed together in a dish, rather than ICSI where a single sperm is injected.

Normal fertilization is judged at the Day 1 check, roughly 17 hours after insemination, by counting pronuclei (PN). A pronucleus is a visible nucleus in the newly fertilized egg, and two of them, 2PN, is the normal result: one from the egg and one from the sperm.

There are three lines:

  • Normal fertilization rate: your monthly rate.

  • Competency: the Vienna Consensus competency level, 60% or above.

  • Benchmark: the Vienna Consensus benchmark, 75% or above.

Reading the card

  • The denominator is the eggs placed in the IVF dish for the cycle, not counting any egg that was later discarded. The numerator is those graded 2PN at the Day 1 check.

  • Discarded eggs are left out of both sides of the rate. Because eggs that failed to fertilize are the ones most often discarded, this card reads higher than a count that kept them in.

  • The Competency and Benchmark lines are flat reference lines from the Vienna Consensus, not measurements from your lab.

  • Hover any point to see the exact figure.

  • The strict Vienna definition of normal fertilization also requires two polar bodies to be visible alongside the two pronuclei. Polar body counts are not reliably recorded, so this card uses the two-pronuclei observation on its own. That makes the rate read very slightly high compared with a strict count.

  • Only eggs that were actually graded on Day 1 can be counted as normal. Where the Day 1 check goes unrecorded, this rate reads low for a recording reason rather than a clinical one. The Day 1 grading coverage gap card on the same tab tells you how much of that is happening.

The card lives on the Laboratory performance (Vienna consensus KPIs) dashboard, on the Fertilization Performance tab, and responds to the filters at the top:

  • Date: change the time window. It scopes by the date of fertilization, and defaults to the past 6 months plus the current month.

  • Clinic: focus on one or more locations, if your organization has several.

  • Treatment type, Age at cycle start and BMI: narrow to a subset of cycles.

Read this next to 1PN rate (IVF), IVF polyspermy rate and Failed fertilization rate (IVF) on the same tab. Between them they account for the eggs that did not fertilize normally.

Where this data comes from

Both halves of this card come from the Culture tab of the patient's Cycle page: the insemination is recorded as a fertilization event, and the Day 1 observation on each egg carries the pronuclei grading.

Recording the Day 1 check on every inseminated egg, including the ones that did not fertilize, is what makes this rate accurate.

Opening this card

In wawa, open Insights from the sidebar, then go to Standard Reports → Laboratory Performance → Laboratory performance (Vienna consensus KPIs). Select the Fertilization Performance tab. The IVF normal (2PN) fertilization rate card is on it.

Did this answer your question?