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ICSI normal fertilization rate

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Written by Andrew Manderson

What this card shows

The ICSI normal fertilization rate card shows what share of injected oocytes fertilized normally, month by month. ICSI is intracytoplasmic sperm injection, where a single sperm is injected directly into an oocyte.

Normal fertilization is judged at the Day 1 check, roughly 17 hours after injection, by counting pronuclei (PN). A pronucleus is a visible nucleus in the newly fertilized oocyte, and two of them, 2PN, is the normal result: one from the oocyte and one from the sperm.

There are four lines:

  • 2PN rate: your monthly rate.

  • Excluding discarded oocytes: the same rate with discarded oocytes left out.

  • Competency: the Vienna Consensus competency level, 65% or above.

  • Benchmark: the Vienna Consensus benchmark, 80% or above.

Excluding discarded oocytes is the dotted line, shown for contrast. It answers the same question while leaving out any that the lab later discarded.

The two lines together tell you how much of your result rests on oocytes that were discarded afterwards. The headline is the one to read and the one the reference lines apply to, because the Vienna Consensus fixes this denominator at the Day 1 observation, before any disposal decision has been made, and it declines to set benchmarks for measures that depend on what a lab chooses to discard or freeze. The dotted line therefore has no competency or benchmark applied to it.

Reading the card

  • The denominator is all oocytes linked to an ICSI fertilization in the window. Every injected oocyte is treated as a mature oocyte, since the protocol only injects mature ones.

  • The card covers standard ICSI along with the p-ICSI, d-ICSI and IMSI variants. Rescue ICSI is excluded, since it is a salvage procedure on oocytes that already failed to fertilize and would not be a fair comparison.

  • The Competency and Benchmark lines are flat reference lines from the Vienna Consensus, not measurements from your lab.

  • Hover any point to see the exact figure.

Two definitional notes:

  • The strict Vienna definition also requires two polar bodies to be visible alongside the two pronuclei. Polar body counts are not reliably recorded, so this card uses the two-pronuclei observation on its own.

  • Strict Vienna scope is narrower than what is measured here. It excludes oocytes matured in the lab, thawed oocytes, and cycles using surgically retrieved sperm. Those exclusions are not applied, because the underlying records do not identify them, so a lab with a lot of surgical-sperm or in-vitro-matured work may read slightly lower here than against a strict comparator.

Only oocytes that were actually graded on Day 1 can be counted as normal, so where the Day 1 check goes unrecorded this rate reads low for a recording reason rather than a clinical one. The Day 1 grading coverage gap card on the same tab tells you how much of that is happening.

The card lives on the Laboratory performance (Vienna consensus KPIs) dashboard, on the Fertilization Performance tab, and responds to the filters at the top:

  • Date: change the time window. It scopes by the date of fertilization, and defaults to the past 6 months plus the current month.

  • Clinic: focus on one or more locations, if your organization has several.

  • Treatment type, Age at cycle start and BMI: narrow to a subset of cycles.

Where this data comes from

Both halves of this card come from the Culture tab of the patient's Cycle page: the injection is recorded as a fertilization event, and the Day 1 observation on each oocyte carries the pronuclei grading.

Recording the Day 1 check on every injected oocyte, including the ones that did not fertilize, is what makes this rate accurate.

Opening this card

In wawa, open Insights from the sidebar, then go to Standard Reports → Laboratory Performance → Laboratory performance (Vienna consensus KPIs). Select the Fertilization Performance tab. The ICSI normal fertilization rate card is on it.

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