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1PN rate (ICSI)

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Written by Andrew Manderson

What this card shows

The 1PN rate (ICSI) card shows what share of injected eggs showed a single pronucleus at the Day 1 fertilization check, month by month. ICSI is intracytoplasmic sperm injection, where a single sperm is injected directly into an egg.

A pronucleus (PN) is a visible nucleus in the newly fertilized egg. Two of them, 2PN, is the normal result. Just one, 1PN, is an abnormal outcome: something has gone wrong in fertilization, and only a small share of these eggs turn out to carry a normal chromosome complement.

There are two lines:

  • 1PN rate: your monthly rate.

  • Benchmark: the Vienna Consensus benchmark, below 3%.

This is one of the cards where lower is better, so you want your line sitting below the benchmark.

Reading the card

  • The denominator is all eggs linked to an ICSI fertilization in the window. Every injected egg is treated as a mature egg, since the protocol only injects mature ones.

  • The card covers standard ICSI along with the p-ICSI, d-ICSI, IMSI and rescue ICSI variants.

  • The Benchmark line is a flat reference line from the Vienna Consensus, not a measurement from your lab.

  • Hover any point to see the exact figure.

  • Because the rate is normally low, single months can look volatile when the number of injected eggs is small. Read the trend across several months.

A sustained rise above the benchmark is the signal to act on. In ICSI, only around one in ten 1PN eggs turns out to be chromosomally normal, and a rising rate points to something in gamete handling, egg activation or culture conditions rather than to chance.

The card lives on the Laboratory performance (Vienna consensus KPIs) dashboard, on the Fertilization Performance tab, and responds to the filters at the top:

  • Date: change the time window. It scopes by the date of fertilization, and defaults to the past 6 months plus the current month.

  • Clinic: focus on one or more locations, if your organization has several.

  • Treatment type, Age at cycle start and BMI: narrow to a subset of cycles.

Read this next to ICSI normal fertilization rate on the same tab, which is the same denominator scored for the normal outcome.

Where this data comes from

Both halves of this card come from the Culture tab of the patient's Cycle page: the injection is recorded as a fertilization event, and the Day 1 observation on each egg carries the pronuclei grading.

Recording the pronuclei count on every injected egg, including the abnormal ones, is what makes this rate accurate. An ungraded egg counts in the denominator but can never appear in the numerator, so gaps in recording push this rate down rather than up.

Opening this card

In wawa, open Insights from the sidebar, then go to Standard Reports → Laboratory Performance → Laboratory performance (Vienna consensus KPIs). Select the Fertilization Performance tab. The 1PN rate (ICSI) card is on it.

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