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CHLOE Integration Guide

This guide explains Chloe - a software that analyses time-lapse imaging data from IVF incubators - and provides a practical approach to deploying it!

Written by Gabby Adams
Updated this week

The CHLOE integration brings embryo data from the EmbryoScope time-lapse system into Wawa. Once connected, you can link cycles to CHLOE and sync embryo gradings, scores, and images directly into the embryology record.

1. Setting up

Adding a station

A station is a connection to CHLOE. To set one up:

  1. Go to 'Settings > CHLOE'

  2. Add a new station

  3. Select your region (US, EU, or AU)

  4. Enter your CHLOE username and password

  5. Click 'Test Connection' to verify everything works

Authentication is handled automatically. Once credentials are entered, wawa manages the connection in the background.

Before you start

Before linking a cycle to CHLOE, make sure the cycle has:

  1. An aspiration recorded in wawa

  2. Fertilised embryos β€” the embryos that will be matched to CHLOE wells must already be marked as fertilised

The sync matches fertilised embryos to wells by position, so these need to exist before you link and sync.

2. Linking a cycle

Once the aspiration and fertilised embryos are in place, you can link the cycle to the corresponding patient and treatment in Chloe.

  1. Open the cycle and go to the CHLOE section

  2. Confirm the patient - wawa shows the patient's name and date of birth from CHLOE so you can verify it's the right person

  3. Choose the treatment - wawa lists all treatments for that patient with their start date and status

  4. Click 'Link'

If anything goes wrong during linking, nothing is saved - you can safely try again!

Syncing embryo data

Once a cycle is linked, you can pull the latest data from CHLOE at any time.

  1. Open the linked cycle and go to the CHLOE section

  2. Click 'Sync'

You can sync as many times as you like. It will always reflect the latest data from CHLOE.

3. What gets synced?

  • Embryo matching: wawa matches fertilised embryos to CHLOE wells by position. The first fertilised embryo is matched to well 1, the second to well 2, and so on.

  • Scores and details: for each matched embryo, the sync brings in:

    • EQ score, blast score, and EQ rank

    • Morphokinetic events (timed cell division milestones)

    • DUC1 and degenerated oocyte flags

    • Fragmentation data

    • Any manual annotations made in Chloe

  • Gradings: CHLOE is treated as the source of truth for gradings. Each sync updates them to reflect the latest data, including any AI re-analysis or manual overrides made in CHLOE. The grading types brought in are:

| Grading type | Days |

|--------------|------|

| PN | Day 1 |

| ASEBIR | Days 1–7 |

| Cleavage | Days 2–4 |

| Gardner | Days 5–7 |

  • Embryo fate: if an embryo's fate hasn't been set manually in wawa, the sync updates it based on the CHLOE well status:

| Chloe status | Wawa fate |

|--------------|-----------|

| Transferred | Transferred |

| Frozen | Cryopreserved |

| Discarded | Discarded |

  • Images: one image per development day is downloaded from CHLOE and attached to the embryo record.

4. If something goes wrong

If one embryo fails to sync, the rest still complete. The error is logged and can be reviewed in the CHLOE logs (under Settings > CHLOE > Logs).

5. Viewing well details

After syncing, you can view the full CHLOE data for any embryo by clicking on its well detail. This shows:

  • Overview: EQ score, blast score, rank, and flags

  • Morphokinetics: Timed events grouped by development day

  • Images: Downloaded embryo images from each day

6. Unlinking a cycle

If you need to disconnect a cycle from CHLOE:

  1. Open the cycle and go to the CHLOE section

  2. Click 'Unlink'

This removes all synced data:

  • CHLOE scores and well details

  • Downloaded images

  • Gradings are reset to blank

The patient link is kept, so if you re-link the cycle later you won't need to enter the patient ID again!

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